Clinical Chemistry
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Clinical Chemistry 23: 1640-1643, 1977;
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Clinical Chemistry, Vol 23, 1640-1643, Copyright © 1977 by American Association for Clinical Chemistry

Determination of morphine by electron capture gas-liquid chromatography

G Nicolau, G Van Lear, B Kaul and B Davidow

We describe a micromethod for determining as little as 0.1 microgram of morphine per liter of human urine. The procedure is about 20-fold more sensitive than are currently used gas-liquid chromatographic methods. It is particularly suitable for use as a confirmatory method for large- scale radioimmunoassay screening tests. The procedure involves extraction of morphine from urine and analysis of its heptafluorobutyryl derivative by gas-liquid chromatography with electron capture detection. Morphine can be determined by this procedure over a range of 0.1-200 microgram/liter of urine, 2 pg of the pure derivative being the lowest amount detectable. Urine samples from patients receiving methadone were analyzed by thin-layer chromatography, radioimmunoassay, and our procedure. The results by our procedure agreed well with those obtained by radioimmunoassay.




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Copyright © 1977 by the American Association for Clinical Chemistry.