Clinical Chemistry
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Clinical Chemistry 24: 115-118, 1978;
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Clinical Chemistry, Vol 24, 115-118, Copyright © 1978 by American Association for Clinical Chemistry

Faster radioimmunoassay of angiotensin I at 37 degrees C

F Fyhrquist and L Puutula

We applied a 1-h radioimmunoassay incubation at 37 degrees C in determining generated angiotensin I in an assay for plasma renin activity. Under these nonequilibrium conditions, 26% of the 125I- labeled angiotensin I was bound at zero dose of unlabeled angiotensin, as compared to 57% at equilibrium after 18 h at 4 degrees C. Sensitivity and useful range for the standard curve remained unchanged. Blanks were not altered. There was a good (r = .971) correlation between renin values in 120 plasma samples from hypertensive patients as measured with both procedures. With isoelectric focusing, we detected no damage to the labeled angiotensin I during incubation for 1 h at 37 degrees C in the presence of diluted plasma, disodium ethylenediaminetetraacetate, hydroxyquinoline, and neomycin. Analytical recovery of unlabeled angiotensin I added to the assay mixture was 98 +/- 2.3% (mean +/- SD). We conclude that our incubation conditions allow rapid and accurate assay of plasma renin activity to be completed in one working day.


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 Clin. Chem.Home page
F. Fyhrquist and O. Saijonmaa
Plasma Renin Activity: An Assay with Ongoing Clinical Relevance
Clin. Chem., August 1, 2008; 54(8): 1400 - 1400.
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Copyright © 1978 by the American Association for Clinical Chemistry.