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Clinical Chemistry 24: 1674-1679, 1978;
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Clinical Chemistry, Vol 24, 1674-1679, Copyright © 1978 by American Association for Clinical Chemistry

Automated metabolic profiling of organic acids in human urine. I. Description of methods

SC Gates, N Dendramis and CC Sweeley

We describe a complete procedure for separation and mechanized analysis of organic acids in human urine. The acid fraction of urine is separated by anion-exchange chromatography on diethylaminoethyl- Sephadex. Individual acids are identified and measured by use of a gas- chromatography/mass spectrometer/computer system that can clearly distinguish contributions from at least 150 substances in a single sample. We discuss analytical recoveries, contributions from the sample separation process, stability of stored samples before and after processing, and reproducibility of the extraction procedure.


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