Clinical Chemistry
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Clinical Chemistry 24: 1711-1719, 1978;
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Clinical Chemistry, Vol 24, 1711-1719, Copyright © 1978 by American Association for Clinical Chemistry

Evaluation of a glassy-carbon electrode for amperometric detection of selected methylxanthines in serum after their separation by reversed- phase chromatography

EC Lewis and DC Johnson

We examined the relative merits of direct-current, pulse, and differential pulse amperometry at a flow-through, glassy-carbon electrode used in monitoring column chromatography. We give a rapid, simple procedure for determining theophylline (1,3-dimethylxanthine) in 20--100 microliter of blood serum. Interference by 1,7- dimethylxanthine, a metabolite of caffeine, is compared for photometric and amperometric detection, and some advantages of combined photometric and amperometric detection are discussed.




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Copyright © 1978 by the American Association for Clinical Chemistry.