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Clinical Chemistry 24: 1738-1739, 1978;
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Clinical Chemistry, Vol 24, 1738-1739, Copyright © 1978 by American Association for Clinical Chemistry

Thyroid hormone contamination in crystalline diiodotyrosine

NM Alexander and M Nishimoto

Crystalline preparations of diiodotyrosine contain trace quantities of thyroxine that can be detected by several competitive radioassays and by chromatography on Sephadex. Spontaneous coupling of diiodotyrosine to thyroxine during iodination of tyrosine and coprecipitation of hormone with diiodotyrosine crystals are likely explanations for the trace contamination. We discuss the significance of these findings in relation to studies on the biosynthesis of thyroxine.




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Copyright © 1978 by the American Association for Clinical Chemistry.