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Clinical Chemistry 24: 1767-1760, 1978;
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Clinical Chemistry, Vol 24, 1767-1760, Copyright © 1978 by American Association for Clinical Chemistry

Use of polyethylene glycol in radioimmunoassay of human placental lactogen

CL Ermshar and DJ Gusseck

We describe a rapid radioimmunoassay for human placental lactogen in biological fluids, with use of polyethylene glycol to separate free from antibody-bound placental lactogen. The standard curve obtained, easily fitted to a logit-log transformation, is useful over a concentration range of 6 to 400 ug/liter. Within-assay variability is 1.97%, between-assay variability 2.20%. The relation is linear when lactogen added is plotted against that accounted for analytically, the actual recovery being 100.26%.




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Copyright © 1978 by the American Association for Clinical Chemistry.