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Clinical Chemistry 24: 1825-1827, 1978;
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Clinical Chemistry, Vol 24, 1825-1827, Copyright © 1978 by American Association for Clinical Chemistry

A non-barbital buffer for immunoelectrophoresis and zone electrophoresis in agarose gels

JF Monthony, EG Wallace and DM Allen

We describe buffer for both immunoelectrophoresis and zone electrophoresis procedures in agarose gels, Tricine [N- tris(hydroxymethyl)methylglycine] and Tris [2-amino-2-(hydroxymethyl)- 1,3-propanediol] being the main components. This buffer eliminates the procurement cost and inventory control associated with formulation of barbital buffers. We compare it with other common buffer formulations.


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Copyright © 1978 by the American Association for Clinical Chemistry.