Clinical Chemistry
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Clinical Chemistry 24: 1838-1841, 1978;
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Clinical Chemistry, Vol 24, 1838-1841, Copyright © 1978 by American Association for Clinical Chemistry

Simultaneous gas-chromatographic analysis for diazepam and its major metabolite, desmethyldiazepam, with use of double internal standardization

DJ Greenblatt

Electron-capture gas-liquid chromatography was used for simultaneous quantitation of plasma diazepam (I) and its major metabolite, desmethyldiazepam(II). Because concentrations of I can greatly exceed those of II after single doses of I, two benzodiazepine-analog internal standards were added to all samples, thereby allowing reliable quantitation of high concentrations of I and low concentrations of II. After extraction at neutral pH with benzene (containing isoamyl alcohol), the organic extract is evaporated and the residue is reconstituted in a small volume of solvent and chromatographed on a 3% OV-17 column. The sensitivity limits are 2 to 3 ng of I or II per milliliter of original sample, with a CV for identical samples of less than 5%. The applicability of the method to single-dose pharmacokinetic studies of I in humans is illustrated.


The following articles in journals at HighWire Press have cited this article:


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 Arch Gen PsychiatryHome page
C. Salzman, R. I. Shader, D. J. Greenblatt, and J. S. Harmatz
Long v Short Half-life Benzodiazepines in the Elderly: Kinetics and Clinical Effects of Diazepam and Oxazepam
Arch Gen Psychiatry, March 1, 1983; 40(3): 293 - 297.
[Abstract] [PDF]

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 ANN INTERN MEDHome page
D. R. ABERNETHY, D. J. GREENBLATT, and R. I. SHADER
Treatment of Diazepam Withdrawal Syndrome with Propranolol
Ann Intern Med, March 1, 1981; 94(3): 354 - 355.
[Abstract] [PDF]

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 Arch Gen PsychiatryHome page
A. Winokur, K. Rickels, D. J. Greenblatt, P. J. Snyder, and N. J. Schatz
Withdrawal Reaction From Long-term, Low-dosage Administration of Diazepam: A Double-blind, Placebo-Controlled Case Study
Arch Gen Psychiatry, January 1, 1980; 37(1): 101 - 105.
[Abstract] [PDF]


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Copyright © 1978 by the American Association for Clinical Chemistry.