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Clinical Chemistry 24: 1903-1907, 1978;
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Clinical Chemistry, Vol 24, 1903-1907, Copyright © 1978 by American Association for Clinical Chemistry

Enzyme immunoassay for gentamicin

JC Standefer and GC Saunders

We describe a gentamicin assay in which a peroxidase-gentamicin conjugate competes with gentamicin for binding to a gentamicin antibody adsorbed to a polystyrene solid phase. The assay can be completed in 30 min and requires 50 microliter of diluted serum. The precision and accuracy are equivalent to that of a radioimmunoassay technique and the reagents are stable for several months.




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Copyright © 1978 by the American Association for Clinical Chemistry.