Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 24: 1908-1911, 1978;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Trivedi, R. C.
Right arrow Articles by Stong, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Trivedi, R. C.
Right arrow Articles by Stong, L.

Clinical Chemistry, Vol 24, 1908-1911, Copyright © 1978 by American Association for Clinical Chemistry

New enzymatic method for serum uric acid at 500 nm

RC Trivedi, L Rebar, E Berta and L Stong

We describe a manual method, well suited to mechanization, for quantitating serum uric acid at 500 nm. In the assay mixture (0.10 ml of sample and 3.00 ml of reagent) the hydrogen peroxide produced from uric acid by uricase is coupled with p-hydroxybenzoate and 4- aminoantipyrine in the presence of peroxidase to form a colored complex, which is measured. A separate sample blank is obviated by taking an initial absorbance measurement 20 s after the sample is added. The reaction is complete within 5 min; its sensitivity is 0.001 deltaA/mg per liter. Absorbances are linearly related to uric acid concentrations up to 120 mg/liter. Many substances that may be present in normal serum do not interfere, but bilirubin in moderately above- normal concentrations will interfere. The procedure can be modified to largely correct for this, when necessary. The proposed method (y) correlated well (r = 0.979) with the uric acid 293 nm reference method (x) and the relation is described by the equation y = 0.998x + 2.42.


The following articles in journals at HighWire Press have cited this article:


Home page
 Diabetes CareHome page
A. Dehghan, M. van Hoek, E. J.G. Sijbrands, A. Hofman, and J. C.M. Witteman
High Serum Uric Acid as a Novel Risk Factor for Type 2 Diabetes
Diabetes Care, February 1, 2008; 31(2): 361 - 362.
[Abstract] [Full Text] [PDF]

Home page
 StrokeHome page
M. J. Bos, P. J. Koudstaal, A. Hofman, J. C.M. Witteman, and M. M.B. Breteler
Uric Acid Is a Risk Factor for Myocardial Infarction and Stroke: The Rotterdam Study
Stroke, June 1, 2006; 37(6): 1503 - 1507.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Clin. Nutr.Home page
H. R Farshchi, M. A Taylor, and I. A Macdonald
Deleterious effects of omitting breakfast on insulin sensitivity and fasting lipid profiles in healthy lean women
Am. J. Clinical Nutrition, February 1, 2005; 81(2): 388 - 396.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Clin. Nutr.Home page
H. R Farshchi, M. A Taylor, and I. A Macdonald
Beneficial metabolic effects of regular meal frequency on dietary thermogenesis, insulin sensitivity, and fasting lipid profiles in healthy obese women
Am. J. Clinical Nutrition, January 1, 2005; 81(1): 16 - 24.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
W. Tang, M. B. Miller, S. S. Rich, K. E. North, J. S. Pankow, I. B. Borecki, R. H. Myers, P. N. Hopkins, M. Leppert, and D. K. Arnett
Linkage Analysis of a Composite Factor for the Multiple Metabolic Syndrome: The National Heart, Lung, and Blood Institute Family Heart Study
Diabetes, November 1, 2003; 52(11): 2840 - 2847.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
N. Raby, C. Bonneau, S. Gillier, J. Le, R. Granouillet, J. Frey, and A. Chamson
Single Dilution for Urine Assays on the Vitros 250 or 700 Analyzers
Clin. Chem., August 1, 1998; 44(8): 1746 - 1748.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1978 by the American Association for Clinical Chemistry.