Clinical Chemistry
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Clinical Chemistry 24: 1990-1995, 1978;
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Clinical Chemistry, Vol 24, 1990-1995, Copyright © 1978 by American Association for Clinical Chemistry

Serum galactose determination with centrifugal analyzers

M Gabrielli

I describe a rapid quantitative centrifugal analysis for galactose in blood. The technique involves galactose dehydrogenase. As compared to the manual (o-toluidine) method for galactose determination, analysis time (4 min 45 s on the GEMSAEC, 4 min 10 s on the CentificCHEM) and sample volume (5 microliter) were considerably reduced. Sample deproteinization is eliminated. A modified commercial kit was also tested. Results by the procedure correlate well with those by the more classical methods and analytical recovery is about 100%. The method is applied to the determination of galactose in intravenous galactose tolerance tests. Results of actual tests are reported.




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Copyright © 1978 by the American Association for Clinical Chemistry.