Clinical Chemistry
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Clinical Chemistry 24: 2033-2035, 1978;
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Clinical Chemistry, Vol 24, 2033-2035, Copyright © 1978 by American Association for Clinical Chemistry

Immobilized-enzyme membrane sandwich reactor used in automated micro- scale assay of plasma uric acid

P Kamoun and O Douay

We describe the automated microassay of plasma uric acid by use of an immobilized uricase-membrane sandwich reactor. Hydrogen peroxide, formed when uric acid is oxidized, oxidatively couples two molecules of p-hydroxyphenylacetic acid in the presence of peroxidase to produce a highly fluorescent compound. The specificity of the uricase reaction is coupled with a significantly lower cost of analysis.




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Copyright © 1978 by the American Association for Clinical Chemistry.