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Clinical Chemistry 24: 2036-2039, 1978;
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Clinical Chemistry, Vol 24, 2036-2039, Copyright © 1978 by American Association for Clinical Chemistry

Continuous-flow analysis for serum albumin, by use of the immediate reaction with bromcresol green

CJ Plesher

Albumin reacts with bromcresol green reagent almost immediately, other serum proteins more slowly. This phenomenon can be exploited to improve the specificity of this routine method for determining serum albumin. I describe a 30-s reaction as adapted to continuous-flow analyzers, with no interference from other serum proteins. The method shows linearity to 72 g/liter. Results of this method correlate very well with those by the Laurell "rocket" and manual (30-s) bromcresol green methods. This procedure gives a considerable improvement in specificity for continuous-flow analyzers as compared with current bromcresol green methods.




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Copyright © 1978 by the American Association for Clinical Chemistry.