Clinical Chemistry
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Clinical Chemistry 24: 2044-2046, 1978;
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Clinical Chemistry, Vol 24, 2044-2046, Copyright © 1978 by American Association for Clinical Chemistry

Enzymatic determination of plasma ammonia: evaluation of Sigma and BMC Kits

M Pesh-Imam, S Kumar and CE Willis

We compared two kit methods for the enzymatic determination of plasma ammonia. The procedures involved measuring the decrease in absorbance at 340 nm resulting from the consumption of NADH in the Sigma kit, NADPH in the BMC kit. Both kits gave acceptable results for aqueous samples, but for plasma specimens values with the Sigma kit significantly exceeded those obtained with the BMC kit. In our hands, values with the BMC kit were more accurate and precise. On the basis of these results, we conclude that the BMC kit is the better choice.




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Copyright © 1978 by the American Association for Clinical Chemistry.