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Clinical Chemistry 24: 2155-2157, 1978;
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Clinical Chemistry, Vol 24, 2155-2157, Copyright © 1978 by American Association for Clinical Chemistry

Radioimmunoassay for urinary lysozyme in human serum from leukemic patients

TL Peeters, YR Depraetere and GR Vantrappen

We present a radioimmunoassay for lysozyme in human serum, based upon human lysozyme isolated from the urine of leukemic patients and antiserum prepared against this lysozyme in the goat. In the separation step, a second antibody is used. By properly adjusting the concentrations of unlabeled and 125I-labeled lysozyme and of the antibodies, maximal precision (SD, 0.04 mg/litre) was obtained in the range 0.00 to 2.00 mg/litre. In 20 normal volunteers the lysozyme concentration was 4.6 +/- 0.8 mg/litre (mean +/- SD), in 13 patients with monocytic leukemia 34.4 +/- 8.6 mg/litre. Correlation with lysoplate determinations was excellent in leukemic sera (r = 0.97) but was poor in normal sera (r = 0.35), possibly owing to the existence of isoenzymes.


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 Clin. Chem.Home page
P. Montagne, M. L. Cuilliere, C. Mole, M. C. Bene, and G. Faure
Microparticle-enhanced nephelometric immunoassay of lysozyme in milk and other human body fluids
Clin. Chem., August 1, 1998; 44(8): 1610 - 1615.
[Abstract] [Full Text] [PDF]


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