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Clinical Chemistry 24: 2166-2168, 1978;
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Clinical Chemistry, Vol 24, 2166-2168, Copyright © 1978 by American Association for Clinical Chemistry

High-performance liquid chromatography of quinidine in plasma, with use of a microparticulate silica column

MA Peat and TA Jennison

We describe a routine method for determining plasma concentrations of quinidine by liquid chromatography. The procedure requires 1.0 ml of plasma (or serum) and involves internal standard addition, extraction with ether, and separation on a column of microparticulate silica. Day- to-day CV (15 days) was less than 5% and no deterioration in column performance has been observed during 12 months. Comparison with a fluorometric procedure gave a correlation coefficient of 0.995.




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Copyright © 1978 by the American Association for Clinical Chemistry.