Clinical Chemistry
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Clinical Chemistry 24: 2178-2179, 1978;
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Clinical Chemistry, Vol 24, 2178-2179, Copyright © 1978 by American Association for Clinical Chemistry

Non-chromatographic screening test for hyperprolinemia

Y Yamaguchi

This procedure for determining serum proline in patients with hyperprolinemia involves protein precipitation (Folin-Wu method), color development with isatin, extraction of the color with methylene chloride and measurement of its absorbance at 600 nm. The specificity and analytical recovery show the method to be suitable for this use.




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Copyright © 1978 by the American Association for Clinical Chemistry.