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Clinical Chemistry 24: 193-199, 1978;
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Clinical Chemistry, Vol 24, 193-199, Copyright © 1978 by American Association for Clinical Chemistry

Method for screening urinary steroids by gas chromatography

P Trocha and NA D'Amato

Numerous methods are available for measuring urinary steroids in evaluating endocrine dysfunctions. Measurements of these particular steroids, or groups of them, usually involve tedious isolation methods, corrections for interferences and losses of the steroid, and (or) expensive reagents. We show how gas-liquid chromatography provides a rapid, sensitive, and direct method for several steroid metabolites in urine. Androstanol, androsterone, etiocholanolone, dehydroisoandrosterone, pregnanediol, pregnanetriol, 11-keto-17- ketosteroids, and the 11beta-hydroxy-17-ketosteroids can be identified and quantified.




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Copyright © 1978 by the American Association for Clinical Chemistry.