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Clinical Chemistry 24: 272-274, 1978;
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Clinical Chemistry, Vol 24, 272-274, Copyright © 1978 by American Association for Clinical Chemistry

Sensitive radioimmunological screening test for antithyroglobulin autoantibodies

P Bodlaender, JR Arjonilla and SL Twomey

The test described here was designed as a screening test to be used in conjunction with the radioimmunoassay for human thyroglobulin. Anti- thyroglobulin autoantibodies, when present even in low concentrations, interfere with thyroglobulin determination. A 30-min incubation allows binding of 125I-thyroglobulin to endogenous anti-thyroglobulin autoantibodies. A subsequent 2-h incubation with goat anti-human gamma- globulin causes precipitation of such complexes. The immunoprecipitable radioactivity reflects the binding capacity of the sample for 125I- labeled thyroglobulin. Samples that are devoid of autoantibodies, and are therefore suitable for valid thyroglobulin determinations, bind less than 6% of the radiolabeled thyroglobulin. The sensitivity of this radioimmunoassay exceeds that of the tanned-erythrocyte hemagglutination test.




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Copyright © 1978 by the American Association for Clinical Chemistry.