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Clinical Chemistry 24: 275-279, 1978;
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Clinical Chemistry, Vol 24, 275-279, Copyright © 1978 by American Association for Clinical Chemistry

Radioimmunoassay of alpha-fetoprotein, with special reference to iodination and purification techniques

HS Schiller, L Kulchinski and DA Luthy

We report a relatively simple and convenient method for iodinating human alpha-fetoprotein and for purifying the 125I-labeled material. The label is incorporated into human alpha-fetoprotein enzymatically by use of lactoperoxidase. Before each assay the labeled material is purified over two successive short columns: Sepharcryl S-200 Superfine and cellulose. This procedure removes both free iodine and "damaged" fetoprotein. With the purified material we developed a sensitive and reliable radioimmunoassay for alpha-fetoprotein in serum and amniotic fluid.




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Copyright © 1978 by the American Association for Clinical Chemistry.