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Clinical Chemistry 24: 309-312, 1978;
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Clinical Chemistry, Vol 24, 309-312, Copyright © 1978 by American Association for Clinical Chemistry

Tartrate-resistant (band 5) acid phosphatase activity measured by electrophoresis on acrylamide gel

WK Lam, LC Lai and LT Yam

A tartrate-resistant acid phosphatase was isolated from a huma spleen infiltrated with reticulum cells of leukemic reticuloendotheliosis. The purified enzyme was used to establish the optimal conditions for quantitative analysis of this enzyme by electrophoresis on acrylamide gel. As little as 0.1 unit of enzyme activity (or 1 ng of the purified enzyme protein) could be quantitatively detected when it was mixed with 4 mg of albumin before electrophoresis.


The following articles in journals at HighWire Press have cited this article:


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 Mol. Pathol.Home page
H Bull, P G Murray, D Thomas, A M Fraser, and P N Nelson
Acid phosphatases
Mol. Pathol., April 1, 2002; 55(2): 65 - 72.
[Abstract] [Full Text] [PDF]

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 Clin. Chem.Home page
A. J. Janckila, K. Takahashi, S. Z. Sun, and L. T. Yam
Tartrate-resistant Acid Phosphatase Isoform 5b as Serum Marker for Osteoclastic Activity
Clin. Chem., January 1, 2001; 47(1): 74 - 80.
[Abstract] [Full Text] [PDF]

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 Clin. Chem.Home page
M. Nakanishi, K. Yoh, T. Miura, T. Ohasi, S. K. Rai, and K. Uchida
Development of a Kinetic Assay for Band 5b Tartrate-resistant Acid Phosphatase Activity in Serum
Clin. Chem., April 1, 2000; 46(4): 469 - 473.
[Abstract] [Full Text] [PDF]

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 Clin. Chem.Home page
M. Nakanishi, K. Yoh, K. Uchida, S. Maruo, and A. Matsuoka
Improved method for measuring tartrate-resistant acid phosphatase activity in serum
Clin. Chem., February 1, 1998; 44(2): 221 - 225.
[Abstract] [Full Text] [PDF]


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Copyright © 1978 by the American Association for Clinical Chemistry.