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Clinical Chemistry, Vol 24, 309-312, Copyright © 1978 by American Association for Clinical Chemistry
WK Lam, LC Lai and LT Yam
A tartrate-resistant acid phosphatase was isolated from a huma spleen infiltrated with reticulum cells of leukemic reticuloendotheliosis. The purified enzyme was used to establish the optimal conditions for quantitative analysis of this enzyme by electrophoresis on acrylamide gel. As little as 0.1 unit of enzyme activity (or 1 ng of the purified enzyme protein) could be quantitatively detected when it was mixed with 4 mg of albumin before electrophoresis.
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M. Nakanishi, K. Yoh, T. Miura, T. Ohasi, S. K. Rai, and K. Uchida Development of a Kinetic Assay for Band 5b Tartrate-resistant Acid Phosphatase Activity in Serum Clin. Chem., April 1, 2000; 46(4): 469 - 473. [Abstract] [Full Text] [PDF] |
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M. Nakanishi, K. Yoh, K. Uchida, S. Maruo, and A. Matsuoka Improved method for measuring tartrate-resistant acid phosphatase activity in serum Clin. Chem., February 1, 1998; 44(2): 221 - 225. [Abstract] [Full Text] [PDF] |
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