Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 24: 362-364, 1978;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Carter, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Carter, P.

Clinical Chemistry, Vol 24, 362-364, Copyright © 1978 by American Association for Clinical Chemistry

Preparation of ligand-free human serum for radioimmunoassay by adsorption on activated charcoal

P Carter

I describe a convenient method for stripping hormones and other ligands from human serum. Formation of a serum/charcoal colloid is avoided by incubating serum on a limited surface area with activated charcoal, in a lateral oscillating shaker. Particle-free serum of negligible turbidity is obtained without ultracentrifugation by ejecting the centrifugate through a cellulose ester membrane. By this technique, more than 80% of thyrotropin, triiodothyronine, thyroxine, cortisol, insulin, digoxin, folate, estriol, or an ether derivative of testosterone is removed from serum. About 70% of follitropin and somatotropin are removed, as well as half the choriogonadotropin. Serum protein remains intact throughout the adsorption process.


The following articles in journals at HighWire Press have cited this article:


Home page
 EndocrinologyHome page
D. R. Goldman-Johnson, D. M. de Kretser, and J. R. Morrison
Evidence that Androgens Regulate Early Developmental Events, Prior to Sexual Differentiation
Endocrinology, January 1, 2008; 149(1): 5 - 14.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1978 by the American Association for Clinical Chemistry.