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Clinical Chemistry 24: 376-378, 1978;
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Clinical Chemistry, Vol 24, 376-378, Copyright © 1978 by American Association for Clinical Chemistry

Determination of 5-hydroxytryptophan in plasma by high-performance liquid chromatography and fluorometric detection after phthaldialdehyde reaction

F Enbaek and I Magnussen

We describe the sensitive and precise determination 5-hydroxytryptophan in plasma from patients treated with this compound. The method is based on solvent extraction of plasma, high-performance liquid chromatography and continuous fluorometric detection after reaction with phthaldialdehyde in strong acid. The combined time for chromatography and reaction is 25 min. The sensitivity of the method is adequate to quantitate 0.05 mumol of 5-hydroxytryptophan per liter, in 750 microliter of plasma. No interference by other hydroxyindole compounds was found.




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Copyright © 1978 by the American Association for Clinical Chemistry.