Clinical Chemistry
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Clinical Chemistry 24: 422-428, 1978;
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Clinical Chemistry, Vol 24, 422-428, Copyright © 1978 by American Association for Clinical Chemistry

Radioimmunoassay of creatine kinase isoenzymes in human serum: isoenzyme BB

MH Zweig, AC Van Steirteghem and AN Schechter

We describe a sensitive, specific radioimmunoassay for the BB isoenzyme of creatine kinase (CK-BB) in serum. A sequential saturation assay was used to achieve sufficient sensitivity to detect the isoenzyme in 100- microliter serum samples of all healthy persons and patients tested. Bound and free antigen were separated by a second antibody system. Large excesses of purified isoenzyme MM did not react in the assay. Cross reactivity of two preparations of CK-MB was only 1 to 7+. The 95th percentile of serum CK-BB in 208 healthy adults was 6.2 microgram/liter. Within-assay and between-assay precision ranged from 5.5 to 11.9% and 9.7 to 13.6%, respectively.


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Home page
 Arch Gen PsychiatryHome page
M. H. Zweig, A. C. Van Steirteghem, and E. F. Torrey
Creatine Kinase Isozymes in the Serum and CSF of Schizophrenic Patients
Arch Gen Psychiatry, October 1, 1981; 38(10): 1107 - 1109.
[Abstract] [PDF]


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Copyright © 1978 by the American Association for Clinical Chemistry.