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Clinical Chemistry 24: 471-474, 1978;
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Clinical Chemistry, Vol 24, 471-474, Copyright © 1978 by American Association for Clinical Chemistry

Flameless atomic absorption spectrophotometric determination of manganese in rat brain and other tissues

E Bonilla

I describe a method for determining manganese in biological tissues by flameless atomic absorption spectrophotometry, It is simple to perform and does not require prior extraction with solvents. Small tissue samples are dried for 48 h at 110 degrees C and dissolved in concentrated nitric acid; the clear acid solution is introduced into the furnace and the sequential drying (120 degrees C, 20 s), charring (800 degrees C, 70 s), and atomization (2400 degrees C, 8 s) are automatically performed. The technique of standard additions was used ot overcome matrix effect. The detection limit is 0.86 X 10(-11) g of Mn. The CV is 4.7%. Only sodium and potassium interfere at 500-fold concentrations, and their interferences were overcome by using the Deuterium Arc Background Corrector.




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Copyright © 1978 by the American Association for Clinical Chemistry.