Clinical Chemistry
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Clinical Chemistry 24: 475-479, 1978;
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Clinical Chemistry, Vol 24, 475-479, Copyright © 1978 by American Association for Clinical Chemistry

Direct assays of lactate, pyruvate, beta-hydroxybutyrate, and acetoacetate with a centrifugal analyzer

JL Hansen and EF Freier

Methods are described for direct assays of lactate, pyruvate, beta- hydroxybutyrate, and acetoacetate in plasma with the GEMSAEC centrifugal analyzer. The methods for lactate, beta-hydroxybutyrate, and acetoacetate are kinetic and ratiometric, eliminating the need for specimen-blank assays. The pyruvate method is an end-point assay, because endogenous lactate dehydrogenase interferes in a kinetic pyruvate assay. The methods are precise and accurate and 1-min of analysis time is adequate for each assay. Rapid assessment and monitoring of metabolic acidosis is possible with these methods, as is illustrated by examples.


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