Clinical Chemistry
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Clinical Chemistry 24: 702-705, 1978;
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Clinical Chemistry, Vol 24, 702-705, Copyright © 1978 by American Association for Clinical Chemistry

Albumin activation of urinary amylase as determined with the Du Pont aca

CC Garber and RN Carey

Protein activation of urinary alpha-amylase (EC 3.2.1.1) activity was observed during an evaluation of the Du Pont aca procedure for the determination of urinary alpha-amylase. This activation effect became constant for urinary albumin concentrations exceeding 1.50 g/liter. It is recommended that urinary alpha-amylase be analyzed with sufficient albumin added to maximize this effect. The aca alpha-amylase procedure is compared to an amyloclastic method for both serum and urine analysis. Expected ranges are presented for the aca method for serum and urinary amylase, amylase clearance, and the amylase clearance/creatinine clearance ratio.




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Copyright © 1978 by the American Association for Clinical Chemistry.