Clinical Chemistry
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Clinical Chemistry 24: 826-827, 1978;
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Clinical Chemistry, Vol 24, 826-827, Copyright © 1978 by American Association for Clinical Chemistry

N-Ethylmaleimide prevents destruction of corticotropin (ACTH) in plasma

W Jubiz and G Nolan

Addition of N-ethylmaleimide, an inhibitor of proteolytic enzymes, to samples for plasma corticotropin determinations prevents destruction of the hormone at room temperature for at least 72 h. A concentration of 125 mg per liter of blood is effective. N-Ethylmaleimide is not so effective in preventing corticotropin degradation in whole blood. Use of the inhibitor should make plasma corticotropin determinations more practical and reliable.




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Copyright © 1978 by the American Association for Clinical Chemistry.