Clinical Chemistry
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Clinical Chemistry 24: 1144-1146, 1978;
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Clinical Chemistry, Vol 24, 1144-1146, Copyright © 1978 by American Association for Clinical Chemistry

Simultaneous determination of phosphatidylglycerol and the lecithin/sphingomyelin ratio in amniotic fluid

GR Gotelli, RE Stanfill, PM Kabra, FA Farina and LJ Marton

We describe a single-dimension thin-layer chromatographic method by which one can simultaneously determine the lecithin/sphingomyelin ratio and the proportion of phosphatidylglycerol in amniotic fluid. The phospholipids are conveniently detected by an improved molybdenum-blue spray reagent, which immediately produces blue spots on a white background. The stained phospholipids are stable for at least 90 min after spraying, and densitometry results in symmetrical peaks that are easily quantitated.





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Copyright © 1978 by the American Association for Clinical Chemistry.