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Clinical Chemistry 24: 1485-1490, 1978;
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Clinical Chemistry, Vol 24, 1485-1490, Copyright © 1978 by American Association for Clinical Chemistry

Determination of aluminum in biological samples by atomic absorption spectrophotometry with a graphite furnace

JE Gorsky and AA Dietz

Aluminum, generally considered non-essential and non-toxic, may accumulate in toxic amounts in the brain in cases of chronic renal failure. We describe a procedure for its analysis in biological fluids by atomic absorption spectrophotometry with a graphite furnace. No sample preparation is required and the procedure is sensitive at the appropriate concentrations. A sample of serum or urine is pipetted into the interior of the graphite tube, where it is sequentially dried, charred, and atomized. Precautions for sample handling are discussed and instrument settings are defined. Precision and accuracy of the method are evaluated, as are the effects of salts, protein content of serum, and specific gravity of urine. Serum of 23 persons who were not consuming aluminum-containing antacids contained 28 +/- 9 (SD) microgram of Al per liter (1.02 +/- 0.33 mumol/liter).


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Copyright © 1978 by the American Association for Clinical Chemistry.