Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 24: 1504-1508, 1978;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Noma, A.
Right arrow Articles by Okabe, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Noma, A.
Right arrow Articles by Okabe, H.

Clinical Chemistry, Vol 24, 1504-1508, Copyright © 1978 by American Association for Clinical Chemistry

Simultaneous determination of serum cholesterol in high- and low- density lipoproteins with use of heparin, Ca2+, and an anion-exchange resin

A Noma, K Nezu-Nakayama, M Kita and H Okabe

Cholesterol concentrations in serum high-density and low-density lipoproteins are simultaneously determined simply, specifically, and rapidly by use of the precipitation method with heparin, Ca2+, and an anion-exchange resin. The isolation of lipoproteins is reproducible, selective, and complete, as judged by electrophoresis on polyacrylamide gel and by immunoelectrophoresis, with use of samples with very-low- density lipoprotein triglyceride concentrations of less than 3.5 g/liter. The precision of the present method is as good (CV, 2.8-3.1%) as that for the method used by the U.S. Lipid Research Clinics (CV 2.0- 3.2%). The present method and the heparin-Mn2+ method of the Clinics gave results that agreed reasonably well (for low-density-lipoprotein cholesterol r = 0.935, P less than 0.001; for high-density-lipoprotein cholesterol r = 0.837, P less than 0.001). we also describe the relations between high- or low-density lipoprotein cholesterol and total cholesterol, and between cholesterol concentrations in these two lipoprotein classes.


The following articles in journals at HighWire Press have cited this article:


Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
X. P. Yang, A. Inazu, K. Yagi, K. Kajinami, J. Koizumi, and H. Mabuchi
Abetalipoproteinemia Caused by Maternal Isodisomy of Chromosome 4q Containing an Intron 9 Splice Acceptor Mutation in the Microsomal Triglyceride Transfer Protein Gene
Arterioscler Thromb Vasc Biol, August 1, 1999; 19(8): 1950 - 1955.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
S. Jimi, N. Uesugi, K. Saku, H. Itabe, B. Zhang, K. Arakawa, and S. Takebayashi
Possible Induction of Renal Dysfunction in Patients With Lecithin:Cholesterol Acyltransferase Deficiency by Oxidized Phosphatidylcholine in Glomeruli
Arterioscler Thromb Vasc Biol, March 1, 1999; 19(3): 794 - 801.
[Abstract] [Full Text] [PDF]

Home page
 J Am Coll CardiolHome page
K. Saku, B. Zhang, T. Ohta, and K. Arakawa
Quantity and function of high density lipoprotein as an indicator of coronary atherosclerosis
J. Am. Coll. Cardiol., February 1, 1999; 33(2): 436 - 443.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
K. Takata, K. Saku, T. Ohta, M. Takata, H. Bai, S. Jimi, R. Liu, H. Sato, G. Kajiyama, and K. Arakawa
A New Case of ApoA-I Deficiency Showing Codon 8 Nonsense Mutation of the ApoA-I Gene Without Evidence of Coronary Heart Disease
Arterioscler Thromb Vasc Biol, November 1, 1995; 15(11): 1866 - 1874.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1978 by the American Association for Clinical Chemistry.