Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 24: 1515-1517, 1978;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Peter, F.
Right arrow Articles by Strunc, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Peter, F.
Right arrow Articles by Strunc, G.

Clinical Chemistry, Vol 24, 1515-1517, Copyright © 1978 by American Association for Clinical Chemistry

Fluorometric determination of erythrocyte protoporphyrin in blood, a comparison between direct (hematofluorometric) and indirect (extraction) methods

F Peter, G Growcock and G Strunc

We measured the concentration of erythrocyte protoporphyrin in 43 blood samples by two fluorometric methods. In the indirect method, protoporphyrin was extracted from blood and the fluorescence of the free acid in the extract was determined with a filter-fluorometer. These results were compared with results of direct determination of protoporphyrin in the same samples. In the direct assay the fluorescence of protoporphyrin, present in blood as the zinc salt, was measured without extraction, with the use of a hematofluorometer. Comparison of the results of the direct and indirect methods showed an excellent correlation (r = 0.986), but the hematofluorometric values (y) were approximately 9% lower than the fluorometric values (y = 0.911x). The slope of the regression line (0.911) is likely to be different for each hematofluorometer-fluorometer combination. Therefore, hematofluorometric values can only be compared with fluorometric values if the slope of the regression line is known and is used as a correction factor.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1978 by the American Association for Clinical Chemistry.