Clinical Chemistry
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Clinical Chemistry 24: 1539-1542, 1978;
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Clinical Chemistry, Vol 24, 1539-1542, Copyright © 1978 by American Association for Clinical Chemistry

A fluorogenic substrate for angiotensin-converting enzyme in plasma

FS Russo, AV Persson and IB Wilson

A simple, sensitive, and reproducible assay for angiotensin-converting enzyme is described. It is based on the hydrolysis of the minimally fluorescent substrate p-nitrobenzyloxycarbonylglycyl-L- tryptophylglycine to the products p-nitrobenzyloxycarbonylglycine and the highly fluorescent L-tryptophylglycine. The L-tryptophylglycine was analyzed by fluorometry (lambda excitation = 285 nm; lambda emission = 350 nm). The mean value for human plasma (serum) is 16.5 nmol of substrate hydrolyzed per minute per milliter of plasma under the described assay conditions.




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Copyright © 1978 by the American Association for Clinical Chemistry.