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Clinical Chemistry 24: 1609-1611, 1978;
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Clinical Chemistry, Vol 24, 1609-1611, Copyright © 1978 by American Association for Clinical Chemistry

Use of the vertical rotor to facilitate estrogen and progesterone receptor analysis

LF Hofman, C Moline, G McGrath and EJ Barron

Sucrose density-gradient analysis is one method of identifying specific estrogen and progesterone receptors in mammary tumor tissue. Use of the vertical rotor makes this practical for routine applications in the clinical laboratory by increasing the number of samples that can be run at one time and shortening the centrifuge time from 18 to less than 2 h. The separations and reproducibility compare favorably with those obtained with a swinging-bucket rotor.




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Copyright © 1978 by the American Association for Clinical Chemistry.