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Clinical Chemistry 24: 1644-1646, 1978;
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Clinical Chemistry, Vol 24, 1644-1646, Copyright © 1978 by American Association for Clinical Chemistry

Kinetic measurement of alpha-amylase with a centrifugal analyzer

M Iosefsohn and JM Hicks

We compared results of an enzymic assay for alpha-amylase (EC 3.2 1.1), adapted to a centrifugal analyzer (GEMSAEC), with results by the manual dyed-starch (Phadebas) amylase assay. We assessed linearity and precision, and the stability of the reagent. The kinetic procedure is rapid, precise, requires only 25 microliter of serum, and thus is evidently suited for both pediatric and emergency use.





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