Clinical Chemistry
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Clinical Chemistry 25: 141-143, 1979;
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Clinical Chemistry, Vol 25, 141-143, Copyright © 1979 by American Association for Clinical Chemistry

Removal of contaminating substances before gas-liquid-chromatographic determination of aldosterone in urine

V Graef, E Furuya and O Nishikaze

This paper deals with removal of contaminants before gas- chromatographic determination of aldosterone in urine. Urine is incubated with beta-glucuronidase, which hydrolyzes all beta- glucuronides except aldosterone-18-glucuronide. The contaminants (the aglycones released and other methylene chloride-soluble substances) are extracted with methylene chloride. Solvolysis of the aqueous phase liberates aldosterone from aldosterone-18-glucuronide, which then is extracted with methylene chloride and oxidized by use of periodic acid. The resulting lactone can be easily separated by one-dimensional thin- layer chromatography and determined by gas-liquid chromatography.




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Copyright © 1979 by the American Association for Clinical Chemistry.