Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 25: 147-150, 1979;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Urdal, P.
Right arrow Articles by Stromme, J. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Urdal, P.
Right arrow Articles by Stromme, J. H.

Clinical Chemistry, Vol 25, 147-150, Copyright © 1979 by American Association for Clinical Chemistry

Effects of Ca, Mg, and EDTA on creatine kinase activity in cerebrospinal fluid

P Urdal and JH Stromme

For one to obtain a precise estimate of creatine kinase (CK) activity in cerebrospinal fluid, the sample fraction is increased by about 10- fold over that used for serum. This increases the concentration of interfering substances, Ca being especially important. Therefore, the relationship between Ca, Mg, and EDTA was examined. Enzyme activity was maximal with 15 mmol of Mg per liter in the presence of 3 mmol of EDTA per liter, otherwise according to the (Scandinavian) recommended conditions for determination of CK activity in serum. These modifications increased the activity of CK by 35% for CK-MM and by 60% for CK-BB. Counteraction of Ca-induced inhibition was the main reason to this increase. We describe a practical and sensitive method for determining CK in cerebrospinal fluid.


The following articles in journals at HighWire Press have cited this article:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
R. Southworth and P. B. Garlick
Dobutamine responsiveness, PET mismatch, and lack of necrosis in low-flow ischemia: is this hibernation in the isolated rat heart?
Am J Physiol Heart Circ Physiol, June 5, 2003; 285(1): H316 - H324.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pathol.Home page
N Durany, J Carreras, M Valenti, J Camara, and J Carreras
Inactivation of phosphoglycerate mutase and creatine kinase isoenzymes in human serum
Mol. Pathol., August 1, 2002; 55(4): 242 - 249.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
A. K. Snabaitis, M. J. Shattock, and D. J. Chambers
Comparison of Polarized and Depolarized Arrest in the Isolated Rat Heart for Long-term Preservation
Circulation, November 4, 1997; 96(9): 3148 - 3156.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1979 by the American Association for Clinical Chemistry.