Clinical Chemistry
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Clinical Chemistry 25: 156-158, 1979;
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Clinical Chemistry, Vol 25, 156-158, Copyright © 1979 by American Association for Clinical Chemistry

Improved determination of serum theophylline by gas chromatography with use of a nitrogen-phosphorus detector

B Vinet and L Zizian

We describe a method for the 7-min determination of theophylline in less than 100 muL of serum. The procedure requires no centrifugation or solvent evaporation after extraction. Butylation is done on the gas- chromatographic column by injecting the serum extract followed by a butylating mixture which contains 1-iodobutane as the alkylating agent. The method is precise, accurate, and free of interference. Results correlate well with those by ultraviolet spectrophotometry.




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Copyright © 1979 by the American Association for Clinical Chemistry.