Clinical Chemistry
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Clinical Chemistry 25: 20-23, 1979;
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Clinical Chemistry, Vol 25, 20-23, Copyright © 1979 by American Association for Clinical Chemistry

Immobilized enzymes in continuous-flow analysis

M Werner, RJ Mohrbacher, CJ Riendeau, E Murador and S Cambiaghi

Glucose oxidase, uricase, and urease were immobilized on the interior surface of activated polyamide tubing. The shelf-life of such enzyme bearing tubes was at least six months. The tubes were used for continuous-flow analysis of glucose, uric acid, and urea with conventional systems and with hybrid micro-scale systems in which modules of different manufacture were combined. The length of enzyme- bearing tube required for each system was ascertained empirically. Each tube could be used for several thousand assays, but glucose oxidase- bearing tubes were more stable than urease- or uricase-bearing tubes. Results for patients' samples correlated well with results obtained by accepted methods.




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Copyright © 1979 by the American Association for Clinical Chemistry.