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Clinical Chemistry 25: 1700-1703, 1979;
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Clinical Chemistry, Vol 25, 1700-1703, Copyright © 1979 by American Association for Clinical Chemistry

Serum cortisol and 11 deoxycortisol by liquid chromatography: clinical studies and comparison with radioimmunoassay

E Canalis, AM Caldarella and GE Reardon

We describe a liquid-chromatographic procedure for separating and measuring cortisol and 11-deoxycortisol in serum. We quantitated these steroids in patients who were undergoing various tests of pituitary and (or) adrenal function and compared the results with those obtained by two radioimmunoassays done in two different laboratories. Results of 48 tests done in 37 functionally normal humans are presented. Cortisol values for sera collected in the morning as determined by liquid chromatography were (mean +/- SD) 134 +/- 54 micrograms/L. Serum cortisol concentrations increased from 136 +/- 65 to 321 +/- 80 micrograms/L 60 min after injecting synthetic corticotropin and increased from 107 +/- 46 to 242 +/- 31 micrograms/L after insulin- induced hypoglycemia. Serum cortisol decreased from 142 +/- 49 to 26 +/- 20 micrograms/L after oral administration of metyrapone, while 11- deoxycortisol increased from less than 10 to 210 +/- 53 micrograms/L. Serum cortisol measured less than 10 micrograms/L the morning after oral ingestion of dexamethasone. Results of the dynamic tests of adrenal function correlated well with previously reported studies. However, the cortisol values obtained by our technique were generally lower than those obtained by radioimmunoassay, possibly owing to lack of specificity of the latter methods used here for comparison. In contrast, values for 11-deoxycortisol were the same by both methods. The present studies confirm the usefulness of liquid chromatography for measuring these two steroids in serum during tests of pituitary and adrenal function. Future refinements of the technique should continue to increase its clinical applications.


The following articles in journals at HighWire Press have cited this article:


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G. P. Chrousos, L. O'Dowd, T. Uryniak, B. Simpson, F. Casty, and M. Goldman
Basal and Cosyntropin-Stimulated Plasma Cortisol Concentrations, as Measured by High-Performance Liquid Chromatography, in Children Aged 5 Months to Younger than 6 Years
J. Clin. Endocrinol. Metab., June 1, 2007; 92(6): 2125 - 2129.
[Abstract] [Full Text] [PDF]

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E. J. Nye, J. E. Grice, G. I. Hockings, C. R. Strakosch, G. V. Crosbie, M. M. Walters, and R. V. Jackson
Comparison of Adrenocorticotropin (ACTH) Stimulation Tests and Insulin Hypoglycemia in Normal Humans: Low Dose, Standard High Dose, and 8-Hour ACTH-(1-24) Infusion Tests
J. Clin. Endocrinol. Metab., October 1, 1999; 84(10): 3648 - 3655.
[Abstract] [Full Text] [PDF]


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Copyright © 1979 by the American Association for Clinical Chemistry.