Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 25: 1939-1943, 1979;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Toffaletti, J.
Right arrow Articles by Bowers, G. N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Toffaletti, J.
Right arrow Articles by Bowers, G. N., Jr

Clinical Chemistry, Vol 25, 1939-1943, Copyright © 1979 by American Association for Clinical Chemistry

Improvements in and clinical utility of a continuous-flow method for routine measurement of dialyzable (ultrafiltrable) calcium

J Toffaletti and GN Bowers Jr

We describe modifications to the original continuous-flow procedure for dialyzable calcium (Clin. Chem. 23: 1258, 1977) needed to make the method more suitable for routine clinical laboratory use. The modifications simplify the continuous-flow (AutoAnalyzer) manifold, decrease baseline noise, increase the sensitivity, and permit use of a less-expensive fluorometer. Bias due to variation in serum processing is minimized by use of serum samples minimally exposed to air and a pH 7.40 buffer in place of the routinely processed sera and pH 7.30 buffer used formerly. Day-to-day precision (CV) during the past year for samples that included three different lots of quality-control sera was 2 to 3%. The analysis requires 200 micro L of serum, collected with minor additional precautions. We find that dialyzable calcium can be dependably measured in the routine service laboratory and show how this information is clinically more useful than is information on total calcium in serum.


The following articles in journals at HighWire Press have cited this article:


Home page
 J. Exp. Biol.Home page
H. Elofsson, K. J. W. Van Look, K. Sundell, H. Sundh, and B. Borg
Stickleback sperm saved by salt in ovarian fluid
J. Exp. Biol., November 1, 2006; 209(21): 4230 - 4237.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1979 by the American Association for Clinical Chemistry.