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Clinical Chemistry 25: 269-272, 1979;
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Clinical Chemistry, Vol 25, 269-272, Copyright © 1979 by American Association for Clinical Chemistry

Lactate-to-pyruvate or pyruvate-to-lactate assay for lactate dehydrogenase: a re-examination

BF Howell, S McCune and R Schaffer

The pyruvate-to-lactate assay for determining lactate dehydrogenase (EC 1.1.1.27) can now yield linearity equal to or better than that obtained by the lactate-to-pyruvate assay. In addition, there are significant advantages to the pyruvate-to-lactate reaction: (a) a greater change in absorbance per unit time, which allows more accurate spectrophotometric readout; (b) lower reactant concentrations are required, which substantially reduces the cost per assay; (c) solid reagents are used to prepare the assay solution; and (d) reagent solutions are more stable. However, impurities present in commercial NADH preparations may substantially affect measured lactated dehydrogenase activities; therefore, a Standard Reference Material for NADH is being developed for issuance by the National Bureau of Standards.


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Copyright © 1979 by the American Association for Clinical Chemistry.