Clinical Chemistry
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Clinical Chemistry 25: 401-404, 1979;
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Clinical Chemistry, Vol 25, 401-404, Copyright © 1979 by American Association for Clinical Chemistry

Liquid-chromatographic analysis for methylphenidate (Ritalin) in serum

SJ Soldin, YP Chan, BM Hill and JM Swanson

We describe a "high performance" liquid chromatographic method for quantitating methylphenidate in serum. The internal standard, 4,5- diphenylimidazole, and serum or plasma sample are extracted in chloroform, evaporated, and redissolved in 20 mmol/L potassium phosphate (pH 3.5)/high-purity acetonitrile, 80/20 by vol. A centrifuged aliquot is chromatographed on mu-Bondapak C-18 with the phosphate/acetonitrile solvent as mobile phase, a flow rate of 1.6 mL/min, and a column temperature of 40 degrees C. Absorbances are read at 192 nm. This method reliably measures concentrations greater than 20 micrograms/L and has analytical recoveries of 74%.




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Copyright © 1979 by the American Association for Clinical Chemistry.