Clinical Chemistry
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Clinical Chemistry 25: 473-475, 1979;
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Clinical Chemistry, Vol 25, 473-475, Copyright © 1979 by American Association for Clinical Chemistry

The elution profile of vitamin D2 metabolites from sephadex LH20 columns

AW Hay and G Jones

A metabolite of vitamin D2 in the tissues of primates fed this vitamin co-migrates to a significant extent with 24,25-dihydroxyvitamin-D3 on columns of Sephadex LH20, with chloroform/n-hexane (60/40 by vol) as eluent. In individuals receiving vitamin D2 and in individuals with scant exposure to sunlight this metabolite, which we believe to be 25,26-dihydroxyvitamin-D2, may yield falsely higher concentrations of apparent 24,25-dihydroxyvitamin-D3 if separation on LH20 is used.




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Copyright © 1979 by the American Association for Clinical Chemistry.