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Clinical Chemistry 25: 665-668, 1979;
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Clinical Chemistry, Vol 25, 665-668, Copyright © 1979 by American Association for Clinical Chemistry

A simple, direct radioimmunoassay for plasma cortisol, featuring a 125I radioligand and a solid-phase separation technique

D Riad-Fahmy, GF Read, SJ Gaskell, J Dyas and R Hindawi

A simple, direct radioimmunoassay for cortisol in human serum and plasma is described. An antiserum, raised in sheep to a cortisol-3-(O- carboxymethyl)oxime/bovine serum albumin conjugate, is coupled to microcellulose. No extraction is required because plasma samples and standards are incubated with the antiserum and an 125I radioligand in a low-pH buffer, which denatures cortisol-binding globulins. The assay satisfies accepted validation criteria. In addition, results from the radioimmunoassay compare well with those obtained by a gas chromatographic-mass spectrometric technique (r = 0.968; FRIA = 0.97 FGCMS + 2.0 nmol/L). The latter procedure features the very high intrinsic specificity obtained by selected ion monitoring at high mass- spectrometric resolution (M/deltaM = 8500) with a Varian MAT-731 instrument. The simplicity of the radioimmunoassay procedure, with use of reagents prepared "in house," makes this a very practical and economical assay for use in the medium or large endocrine laboratory.


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Copyright © 1979 by the American Association for Clinical Chemistry.