Clinical Chemistry
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Clinical Chemistry 25: 914-917, 1979;
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Clinical Chemistry, Vol 25, 914-917, Copyright © 1979 by American Association for Clinical Chemistry

A direct radioimmunoassay of serum cortisol with in-house 125I-tracer and preconjugated double antibody

PY Wong, AV Mee and FF Ho

We describe a radioimmunoassay of serum cortisol in which 125I-tracer and preconjugated double antibody are used. This simpler method involves direct serum assay, and the majority of reagents are prepared in-house. Within-assay precision (coefficient of variation) of assay of Ortho Diognostic RIA Cortisol I and II was 3.2% (mean = 131 microgram/L, n = 10) and 2.2% (mean = 405 microgram/L, n = 10), respectively. Between-assay precision (coefficient of variation) for the same controls was 6.7% (mean = 128 microgram/L, n = 62) and 5.6% (mean = 395 microgram/L, n = 62), respectivley. By using automatic pipetters and a gamma counter with a data-reduction package, a technologist can complete 100 assays in less than 3 h. The operating cost is approximately 10 cents per tube.


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 Arch Gen PsychiatryHome page
S. H. Kennedy, P. E. Garfinkel, V. Parienti, D. Costa, and G. M. Brown
Changes in Melatonin Levels but Not Cortisol Levels Are Associated With Depression in Patients With Eating Disorders
Arch Gen Psychiatry, January 1, 1989; 46(1): 73 - 78.
[Abstract] [PDF]


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Copyright © 1979 by the American Association for Clinical Chemistry.