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Clinical Chemistry 25: 939-942, 1979;
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Clinical Chemistry, Vol 25, 939-942, Copyright © 1979 by American Association for Clinical Chemistry

Determination of high-density lipoproteins: screening methods compared

GM Kostner, P Avogaro, GB Bon, G Cazzolato and GB Quinci

Factors reflecting the concentration of high-density lipoproteins in serum were assessed for 108 men and 106 women participating in a Venetian screening program for hyperlipoproteinemia. The methods applied, optimized in our laboratory, were: (a) cholesterol in high- density lipoproteins, determined in the supernate after sedimentation of the very-low-density lipoproteins + low-density lipoproteins with dextran sulfate or sodium phosphotungstate; and (b) immunochemical quantitation of apolipoprotein A-I and apolipoprotein A-II by Laurell's "rocket" technique. The latter determinations were performed with total serum before and after delipidation with diispropyl ether/n-butanol (6/4 by vol). The dextran sulfate method gave about 5% higher values than did the phosphotungstate method, but the correlation between the two was excellent (r = 0.95). Results of the immunochemical quantitation indicate that delipidation of lipoproteins before Laurell electrophoresis may not be necessary if only freshly drawn sera are used.


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Home page
 J. Lipid Res.Home page
G. Bittolo-Bon and G. Cazzolato
Analytical capillary isotachophoresis of total plasma lipoproteins: a new tool to identify atherogenic low density lipoproteins
J. Lipid Res., January 1, 1999; 40(1): 170 - 177.
[Abstract] [Full Text]


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Copyright © 1979 by the American Association for Clinical Chemistry.