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Clinical Chemistry 25: 1306-1308, 1979;
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Clinical Chemistry, Vol 25, 1306-1308, Copyright © 1979 by American Association for Clinical Chemistry

Evaluation of a solid-phase enzyme immunoassay for insulin in human serum

K Kato, Y Umeda, F Suzuki, D Hayashi and A Kosaka

We describe a "sandwich-type" enzyme immunoassay for insulin in serum, in which antibody Fab'-beta-D-galactosidase conjugate and an antibody- immobilized silicone rubber solid-phase are used. The interference by serum factors with the solid-phase enzyme immunoassay can now be removed by using a buffer containing gelatin. Serum samples of 50 microL can be analyzed by the enzyme immunoassay, which is as sensitive as radioimmunoassay for human insulin. Our results correlate well with those for radioimmunoassay (r = 0.97, slope = 0.92, y-intercept = 4.6 milli-int. units /L for 181 samples). Between-assay and within-assay coefficients of variation are less than 15% over the useful ranges of the assay (5--160 milli-int. units/L).


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Copyright © 1979 by the American Association for Clinical Chemistry.