Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 25: 1596-1599, 1979;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Meriadec, B.
Right arrow Articles by Henry, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Meriadec, B.
Right arrow Articles by Henry, R.

Clinical Chemistry, Vol 25, 1596-1599, Copyright © 1979 by American Association for Clinical Chemistry

A new and universal free/bound separation technique for the "CENTRIA" automated radioimmunoassay system

B Meriadec, JP Jolu and R Henry

A new separation procedure based on the double-antibody technique has been adapted to the CENTRIA System. This procedure is universally applicable and lends itself to easy adaptation to commercial RIA kits in which liquid reagents are used. This second antibody is covalently linked to agarose (Sepharose) and the lyophilized powder is subsequently tableted for easy use on the instrument. The technique was applied to radioimmunoassay for thyrotropin (thyroid stimulating hormone), alpha-fetoprotein, and ferritin. Performance characteristics were as follows: sensitivity 1.5 milliunits/L, 4.5 micrograms/L, and 4.5 micrograms/L, respectively; intrarun precision 1.7, 9, and 3.4%, and interrun precision 7.6, 13, and 14.5%. All three assays were clinically validated.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1979 by the American Association for Clinical Chemistry.