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Clinical Chemistry 25: 1617-1621, 1979;
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Clinical Chemistry, Vol 25, 1617-1621, Copyright © 1979 by American Association for Clinical Chemistry

High-performance liquid chromatography for ultraviolet-absorbing constituents in human urine

H Miyagi, J Miura, Y Takata and S Ganno

Several liquid-chromatographic systems involving anion-exchange resins and linear acetate gradient elution have been reported previously for the analysis of ultraviolet-absorbing constituents in human urine. We describe a high-performance liquid-chromatographic system on which a macroreticular anion-exchange resin, and stepwise elution are used. Separations were more rapid with ammonium chloride/acetonitrile stepwise elution than with acetate stepwise elution, and urine constituents could be detected at 225 nm with the former system. Analytical conditions were convenient and the method has proven to be useful for routine clinical analysis.




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Copyright © 1979 by the American Association for Clinical Chemistry.